atr pathway Activation: Learn the Key Steps in Simple Terms

Date:

Share post:

So, I wanted to mess around with this “ATR pathway” thing I’d heard about. I didn’t really know much about it, just that it had something to do with DNA damage and keeping cells alive. It sounded complicated, but I figured I’d give it a shot.

atr pathway Activation: Learn the Key Steps in Simple Terms

First, I gathered some cells. I’m not gonna lie, I just used some basic ones I had growing in the lab. Nothing fancy, just your run-of-the-mill human cells.

Next, I needed to damage their DNA. I used this stuff that basically zaps the DNA and creates breaks. You know, kinda like giving the cells a really bad sunburn.

Watching it Happen

  • I added the DNA-damaging agent to the cells and let them sit for a while.
  • Then, I used some special dyes that stick to different parts of the cell. These dyes are fluorescent, so they glow under a microscope.
  • I wanted to see if the ATR pathway was actually getting turned on. So I looked for a specific protein called “ATR” (yeah, real original name, I know). If it was glowing, that meant it was active.

I peeked at the cells under the microscope, and guess what? The ATR protein was glowing like a Christmas tree! That meant the pathway was working – the cells were recognizing the DNA damage and trying to fix it.

Checking the Results

I did a lot of repeat, using antibody to check ATR and its downstream proteins’ expression level.

atr pathway Activation: Learn the Key Steps in Simple Terms

It wasn’t exactly a groundbreaking experiment, but it was cool to actually see this complex pathway in action. I learned that even seemingly complicated biological processes can be broken down and observed with the right tools. Plus, it was just plain fun to play around with cells and microscopes. I can say it is done and dusted!

LEAVE A REPLY

Please enter your comment!
Please enter your name here

Related articles

How to enjoy Nice Lyon best simple tips for your trip planning

Last month I decided to finally visit Lyon after dreaming about it for years. Booked my flight kinda...

5 things to know about tigers new pitcher before the next game starts

My Research Kickoff So my Tigers buddy texts me yesterday morning, all hyped about this new pitcher they just...

Best ways to see the isle of man race date and plan your trip today

Deciding to Go I randomly saw Isle of Man TT highlights online last winter. Those bikes flying through tiny...

2017 Braves Season Success? Explore Their Major Highlights Now

Alright, let’s dive into my research dive into that 2017 Braves season. Honestly, I figured I knew baseball,...